brilliant blue R staining solution*electrophoresi

brilliant blue R staining solution*electrophoresi - Names and Identifiers

Name	Brilliant Blue R
Synonyms	C.I. 42660 SERVA BLUE R Acid Blue 83 Page blue 83 ACID BIUE 83 PAGE BLUE 83 Acid Blue 83 Brilliant Blue R SOLAR CYANINE 6B C.I. Acid Blue 83 Brilliant blue R250 Brilliant Blue 6B R STANDS FOR ''REDDISH'' Coomassie brilliant R250 Coomassie brilliant blue R brilliant blue R concentrate Coomassie Brilliant blue R-250 Coomassie?Brilliant Blue R-250 SERVA BLUE R STAINING SOLUTION brilliant blue R (dye content ca.) brilliant blue R-350 phast gel tablets brilliant blue R staining solution*electrophoresi sodium 3-({[(1Z,4Z)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}methylidene)cyclohexa-2,5-dien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl) amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino ]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]- N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt
CAS	6104-59-2
EINECS	228-060-5
InChI	InChI=1/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
InChIKey	NKLPQNGYXWVELD-UHFFFAOYSA-M

brilliant blue R staining solution*electrophoresi - Physico-chemical Properties

Molecular Formula	C45H46N3NaO7S2
Molar Mass	827.99
Melting Point	174-180 °C
Flash Point	14°C
Water Solubility	Soluble in water.
Solubility	H2O: soluble10mg/mL
Vapor Density	28.5 (vs air)
Appearance	Dark Bordeaux Red to Dark Purple Brown Crystalline Powder
Color	Red Brown
BRN	5718025
Storage Condition	room temp
Stability	Light Sensitive
MDL	MFCD00041762
Use	It is used to detect protein bands after electrophoresis. It is 5-10 times more sensitive than naphthol blue black B(Naphthol Blue Black B) and bromophenol (Bromophenol) (0.5 μg/cm2 protein on gel matrix can be detected).

brilliant blue R staining solution*electrophoresi - Risk and Safety

WGK Germany	3
TSCA	Yes
HS Code	32041200

brilliant blue R staining solution*electrophoresi - Reference

Reference

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5. Zhang Xin, Ding baomiao, Jiang Hongbo. Study on the adsorption thermodynamics of macroporous resin on the washing water of black carp surimi [J]. Food Research and Development, 2019, 40(04):51-54.
6. Wu Yifan, GE Wupeng, Liu Keiru, etc. Analysis of differences in nutritional components and whey protein components between sheep milk and other milk species [J]. Dairy Science and Technology, 2019.
7. Chen Yimeng, Tang Shanhu, Li, Si-ning, et al. Physicochemical and textural properties of ultrasonic-assisted papain and fermented yak meat [J]. Food and Fermentation Industries, 2019(23).
8. Li, Li, Feng, Li, Wang, Guangqiang, et al. Extraction, preparation and purification of Dava gum [J]. Journal of Food and Fermentation Technology, 2019, 055(004):52-58.
9. Guo Tianci, Zhao Shilei, Liu Shisheng. Study on hydrolysis of soybean isoflavones from soybean milk by β-glucosidase from bitter almond [J]. Food Research and Development, 2019(12).
10. Huang, Yanming. Study on degradation mechanism and texture protection of Procambarus clarkii at high temperature [D]. Hubei University of Technology, 2020.
11. Chen Zihong, Huang Maokun. Optimization of protein extraction from channel catfish waste by response surface methodology [J]. Journal of Tonghua Teachers College, 2021,42(02):70-75.
12. Liu Qingqing, Yellow River, Luo Yuke, He Yuxin, Yuan Yongjun. Effects of pH shift on physicochemical indexes and emulsifying properties of Zanthoxylum bungeanum seed protein [J]. Journal of Xihua University (Natural Science Edition),2020,39(06):81-86.
13. Zhang Zhao-yun, Zhou Qi-ping, Yan Qi-fu, Zhao Bao-Tang, Shang Qi, Zhang Zhi-hua, Yang Fumin. Optimization of extraction process and determination of antioxidant activity of albumin from quinoa seed of "Chenopodium album no.1" [J]. Food and Fermentation Industry, 2021,47(02):212-219.
14. [IF = 7.514] Jing-Nan Ren et al."Extraction of orange pectin based on the interaction between sodium caseinate and pectin." Food Chem. 2019 Jun;283:265
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16. [IF=6.119] Peng Jin et al."Efficient bioconversion of high-concentration D-fructose into D-mannose by a novel N-acyl-D-glucosamine 2-epimerase from Thermobifida halotolerans."Catal Sci Technol. 2021 Mar;11(5):1922-1930
17. [IF=4.952] Meng Wang et al."Thermomechanical behaviors and protein polymerization in bread dough modified by bran components and transglutaminase."Lwt Food Sci Technol. 2020 Nov;133:109894

brilliant blue R staining solution*electrophoresi - Introduction

Insoluble in cold water, slightly soluble in hot water, bright red light blue, slightly soluble in ethanol, bright blue. When encountering concentrated sulfuric acid, it is orange-red. Its aqueous solution with sodium hydroxide is purple.

Last Update：2022-10-16 17:27:04

brilliant blue R staining solution*electrophoresi - Reference Information

Color index	42660
EPA chemical substance information	information provided by: ofmpeb.epa.gov (external link)
properties	acid blue 83, also known as Coomassie Brilliant Blue R250, is a triarylmethane and Xanthene dye. Acid blue 83 is insoluble in cold water, slightly soluble in hot water is brilliant red blue, slightly soluble in ethanol is brilliant blue. In case of concentrated sulfuric acid, it was orange-red and diluted to blue. Its aqueous solution with sodium hydroxide was purple in color.
Use	acid brilliant blue 6B is used for dyeing wool, silk and nylon, and also for coloring leather, feathers and paper, is a standard blue color. The color light is pure, the freshness is good, the dyeing performance is good, is commonly used brilliant blue variety. biochemical study. For the detection of protein bands after electrophoresis. It is 5-10 times more sensitive than Naphthol Blue Black B and bromophanol (0.5 μg/cm2 protein on gel matrix can be detected). The triphenylmethane dye used for protein analysis in SDS-PAGE can also be widely used in Bradford protein quantitative reaction.
preparation	triarylmethane and Xanthene dyes are generally obtained by oxidation of their leucochromes. The existing industrial processes are based on the oxidation of heavy metal compounds such as sodium dichromate, manganese dioxide or lead dioxide in the presence of complexing agents such as oxalic acid in a stoichiometric manner.
production method	with P-chlorobenzaldehyde, 1-(N-ethylaniline) M-methylbenzenesulfonic acid and p-aminophenethyl ether (p-phenetidine) as raw materials, P-chlorobenzaldehyde is first condensed with 1-(N-ethylaniline)-M-methylbenzenesulfonic acid, then the condensation product is oxidized with potassium dichromate-oxalic acid, and finally the product is condensed with P-aminophenyl ether. After salting out, filtration, drying, grinding to get the finished product.. After 1-(N-ethylaniline) M-methylbenzenesulfonic acid was slurried with water, the pH value was adjusted to about 3, p-chlorobenzaldehyde was added, and the temperature was raised to 95-98 ° C., and the reaction was terminated. After an excess of p-chlorobenzaldehyde is distilled off, it is diluted with water and cooled to below 10 ° C., potassium dichromate, sulfuric acid and oxalic acid are added for oxidation. At the end of the reaction, sulfuric acid was added at 45 °c to precipitate a paste, which was dried under vacuum at 95 °c. P-ethoxyaniline was heated to 70 °c, then the above product was added and the temperature was raised to 100 °c for 3-6h to the end point. Diluted with dilute hydrochloric acid at 70 °c, filtered, washed with hot water and dried to give the product. p-chlorobenzaldehyde is obtained by condensation with N-ethyl-N-(3 '-sulphonylbenzyl) aniline, followed by oxidation with potassium dichromate and subsequent condensation with P-aminophenyl ether.